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A new triterpenoid saponin, named segetoside L, was isolated from the seeds of Vaccaria segetalis. On the basis of spectral data and chemical reaction, its structure was established as 28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl oleanolic acid 3-O-β-D-glucopyranosyl-(1→3)-[ β-D-galactopyranosyl-(1→2)]-β-D-galactopyranoside.