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目的:探讨MAPK/ERK信号传导通路抑制剂PD98059对卵巢癌细胞SKOV3的增殖、侵袭能力的影响。初步探讨阻断MAPK/ERK信号传导通路治疗卵巢癌的可能性。方法:体外培养人上皮性卵巢癌细胞SKOV3,采用CCK-8细胞增殖方法检测卵巢癌细胞SKOV3增殖情况,Western blot检测p-ERK蛋白表达,Transwell细胞侵袭实验检测SKOV3细胞的侵袭能力。结果:CCK-8法显示PD98059在一定范围内能够明显抑制卵巢癌细胞SKOV3的增殖,其作用具有时间依赖性及浓度依赖性,PD98059有效浓度为25μmol/L,在此有效浓度作用下有效时间为24h;Western blot法检测结果显示PD98059能够下调pERK1/2,即抑制ERK1/2磷酸化,从而使得ERK1/2信号传导途径失活;Transwell细胞侵袭实验结果显示PD98059在一定浓度范围内能抑制卵巢癌细胞SKOV3的侵袭与转移能力。结论:PD98059可以通过抑制ERK1/2信号途径,抑制人上皮性卵巢癌SKOV3细胞的增殖、侵袭转移。
Objective: To investigate the effect of PD98059, a MAPK / ERK signal transduction pathway inhibitor, on the proliferation and invasion of ovarian cancer cell line SKOV3. To explore the possibility of blocking the MAPK / ERK signaling pathway in the treatment of ovarian cancer. Methods: The human ovarian epithelial ovarian cancer cell line SKOV3 was cultured in vitro. The proliferation of ovarian cancer cell line SKOV3 was detected by CCK-8 cell proliferation assay. The expression of p-ERK protein was detected by Western blot. The invasion ability of SKOV3 cells was detected by Transwell cell invasion assay. Results: CCK-8 assay showed that PD98059 could significantly inhibit the proliferation of SKOV3 cells in a certain range. The effect of PD98059 was dose-dependent and time-dependent. The effective concentration of PD98059 was 25μmol / L, and the effective time was 24h. The results of Western blot showed that PD98059 could down-regulate pERK1 / 2, that is, ERK1 / 2 phosphorylation, thereby inactivating ERK1 / 2 signal transduction pathway. Transwell cell invasion assay showed that PD98059 could inhibit ovarian cancer in a certain concentration range Invasion and metastasis of SKOV3 cells. Conclusion: PD98059 can inhibit the proliferation, invasion and metastasis of human ovarian epithelial ovarian cancer SKOV3 cells by inhibiting the ERK1 / 2 signaling pathway.