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大鼠饮0.9%NaCl(盐负荷)或禁水(单纯性脱水)分别持续2、4、6天。冷酚法提取心房中总RNA,用α-~(32)P-标记的大鼠心房肽cDNA探针与之杂交。结果表明。心房肽mRNA长约1000碱基,盐负荷和单纯性脱水显著影响心房肽mRNA的含量。盐负荷组分别为对照组的2.4、2.8和2.0倍;单纯性脱水组分别为对照组的2.1、1.6和0.38倍。盐负荷及禁水时血浆[Na~+]显著增加。盐负荷时心房肽mRNA含量变化与血浆[Na~+]平行。结果提示,水盐平衡显著影响心房肽基因表达。
Rats drink 0.9% NaCl (salt load) or water forbidden (simple dehydration) for 2, 4 and 6 days respectively. Total RNA was extracted from the atrium by cold phenol method and hybridized with α-32P-labeled rat atrial-peptide cDNA probe. The results show. Atrial peptide mRNA about 1000 bases, salt load and simple dehydration significantly affect atrial natriuretic peptide mRNA content. Salt loading group were 2.4,2.8 and 2.0 times that of the control group respectively; the simple dehydration group were 2.1,1.6 and 0.38 times of the control group respectively. The plasma [Na ~ +] increased significantly under salt load and without water. Changes of atrial natriuretic peptide mRNA in salt load parallel with plasma [Na ~ +]. The results suggest that water and salt balance significantly affect the atrial natriuretic peptide gene expression.