【摘 要】
：
Nagilactone E (NLE),a natural product with anticancer activities,is isolated from Podocarpus nagi.In this study,we reported that NLE increased programmed death ligand 1 (PD-L1) expressions at both protein and mRNA levels in human lung cancer cells,and enh
【机 构】
：
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences,
论文部分内容阅读
Nagilactone E (NLE),a natural product with anticancer activities,is isolated from Podocarpus nagi.In this study,we reported that NLE increased programmed death ligand 1 (PD-L1) expressions at both protein and mRNA levels in human lung cancer cells,and enhanced its localization on the cell membrane.Mechanistically,NLE increased the phosphorylation and expression of c-Jun,and promoted the localization of c-Jun in the nucleus,while silencing of c-Jun by small interfering RNA (siRNA) reduced NLE-induced PD-L1.Further study showed that NLE activated the c-Jun N-terminal kinases (JNK),the upstream of c-Jun,and its inhibitor SP600125 reversed the NLE-increased PD-L 1.Moreover,NLE-induced PD-L1 increased the binding intensity of PD-1 on the cell surface.In summary,NLE upregulates the expression of PD-L1 in lung cancer cells through the activation of JNK-c-Jun axis,which has the potential to combine with the PD-1/PD-L1 antibody therapies in lung cancer.
其他文献
Diterpenoid lactones (DLs),a group of furan-containing compounds found in Dioscorea bulbifera L.(DB),have been reported to be associated with hepatotoxicity.Different hepatotoxicities of these DLs have been observed in vitro,but reasonable explanations fo
KangFuXinYe (KFX),the ethanol extract of the dried whole body of Periplaneta americana,is a well-known important Chinese medicine preparation that has been used to treat digestive diseases such as gastric ulcers for many years in China.However,its therape
Osthole is observed to have the capacity to treat pulmonary arterial hypertension (PAH) in rats,but molecular mechanism is still unknown.The present study aims to discover therapeutic targets and explore therapeutic mechanism of osthole against PAH from m
Carbon monoxide (CO) as one of the three important endogenously produced signaling molecules,termed as “gasotransmitter,” has emerged as a promising therapeutic agent for treating various inflammation and cellular-stress related diseases.In this review,we
Cucurbitacin E (CuE) is previously reported to exhibit antitumor effect by several means.In this study,CuE acted as a tyrosine kinase inhibitor interfering with the epidermal growth factor receptor/mitogen-activated protein kinase (EGFR/MAPK) signaling pa
Enhanced glucose metabolism is one of the hallmarks of pancreatic cancer.MUC 1,a transmembrane protein,is a global regulator of glucose metabolism and essential for progression of pancreatic cancer.To clarify the role of MUC 1 in glucose metabolism,we kno
Three new secoiridoid glycosides,named lonijapoglycol A (1),aldosecolohanin C (2) and aldosecolohanin B (3),together with three known ones (4-6),have been isolated from the flower the buds of Lonicerajaponica.All the structures were identified by spectros
Adriamycin resistance in HCC seriously hinders the treatment of patients,it is necessary to investigate the mechanisms.Autophagy is involved in adriamycin resistance and JNK2 is related to autophagy.However,whether JNK2 inducing drug resistance though aut
Traditional Chinese medicine (TCM) has been paid much attentions due to the prevention and treatment of steroid hormone disorders.Ginseng,the root of Panax ginseng C.A.Meyer (Araliaceae),is one of the most valuable herbs in complementary and altemative me
Alcoholic liver disease (ALD) has become one of the leading causes of death in the world.Berbamine (BM),a natural product mainly derived from Berberis vulgaris L,possesses multiple bioactivities as a traditional medicine.However,the protective effect of B