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目的 观察钙化血管精氨酸 /NO途径的改变和外源性牛磺酸 (taurine,Tau)对血管钙化及精氨酸 /NO途径的影响。方法 维生素D3 (vitaminD3 ,VitD3 )肌注和尼古丁(nicotine ,Nic)灌胃制备大鼠血管钙化模型 ,检测血管钙含量和血管碱性磷酸酶 (ALP)活性、血浆精氨酸和亚硝酸盐(NO-2 )含量、血管环磷酸鸟苷 (cGMP)含量、血管一氧化氮合酶 (NOS)活性及血管精氨酸 (arginine ,Arg)转运。结果 钙化组 (VDN)大鼠血管钙含量较对照组升高 6 6倍 (P <0 0 1) ,血管ALP活性高 12 6倍 (P <0 0 1) ;血浆NO生成减少 ,血管cGMP水平降低 ,血管NOS活性增高 ,其中cNOS活性高 18 9% (P <0 0 1) ,但精氨酸转运在血管平滑肌和内皮明显减少 (- 6 3 8% ,- 5 5 2 % ,P <0 0 1)。口服牛磺酸治疗的大鼠较单纯VDN组 ,血管钙含量降低 4 9 5 % (P <0 0 1) ,血管ALP活性明显降低 ,血浆NO生成增加 ,血管cGMP含量增加 2 9 1% (P均 <0 0 1) ,血管精氨酸转运在血管平滑肌和内皮分别增加 79 4 %和 5 5 1% (P <0 0 1)。结论 给予外源性牛磺酸可以减轻VitD3 加Nic大鼠的血管钙化程度 ,改善钙化血管L Arg/NOS/NO/cGMP途径紊乱 ;提示牛磺酸对防治动脉粥样硬化等疾病的血管钙化可能具有潜在临床意义
Objective To observe the changes of calcified vascular arginine / NO pathway and the effects of exogenous taurine (Tau) on vascular calcification and arginine / NO pathway. Methods The vascular calcification model was established by intramuscular injection of vitamin D3 and nicotine (Nic) and the contents of calcium in the blood vessels and the activity of alkaline phosphatase (ALP) in the blood were measured. The levels of plasma arginine and nitrite NO-2, cGMP, NOS activity and arginine (Arg) transport. Results Compared with control group, the content of calcium in vascular of calcified group (VDN) was increased by 66% (P <0.01), ALP activity of vascular increased by 126% (P <0.01), NO was decreased and the level of cGMP (P <0.01). However, the activity of cNOS was 18 9% (P <0.01), but arginine transport was significantly decreased in vascular smooth muscle and endothelium (-6.38%, -5.52%, P <0 0 1). Oral administration of taurine significantly reduced the calcium content of blood vessels by 495% (P <0.01), reduced the activity of ALP, increased NO production in plasma and increased the content of cGMP in blood vessels by 291% (P All <0 0 1), vascular arginine transport increased by 74.4% and 55.1% (P <0.01) in vascular smooth muscle and endothelium, respectively. Conclusions Administration of exogenous taurine can reduce the degree of vascular calcification in VitD3 plus Nic group and improve the disorder of L Arg / NOS / NO / cGMP pathway in calcified blood vessels. It suggests that the potential of taurine to prevent vascular calcification in diseases such as atherosclerosis Has potential clinical significance