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为探讨多发性骨髓瘤（ＭＭ）的克隆起源，经形态学及ＡＢＣ法筛选１８例外周血无浆细胞污染的ＭＭ患者，采用多聚酶链反应（ＰＣＲ）技术扩增免疫球蛋白重链ＣＤＲ３区编码基因，并经单链构象多态性（ＳＳＣＰ）法分析其单链构象多态性。经平行检测患者外周血（ＰＢ）和骨髓（ＢＭ）标本，发现１５例ＢＭ及１１例ＰＢ获得预期的克隆特异性ＰＣＲ产物为８０～１１０ｂｐ，其中９例ＰＢ和ＢＭ获得相同的ＰＣＲ产物，８例为１条扩增条带，其ＳＳＣＰ分析可见２条单链，另１例ＰＣＲ产物为２条扩增条带，其ＳＳＣＰ分析可见３条单链，同一患者ＰＢ与ＢＭ标本的单链泳动速度一致。提示至少６０％ＭＭ患者ＰＢ和ＢＭ不仅ＰＣＲ产物一致，其单链构象也一致，研究结果证实了外周血Ｂ细胞参与ＭＭ的发病。
In order to explore the origin of the clones of multiple myeloma (MM), 18 cases of peripheral blood-free plasma cells in MM patients were screened by morphology and ABC method, and the immunoglobulin heavy chain CDR3 region was amplified by polymerase chain reaction The single strand conformation polymorphism was analyzed by single strand conformation polymorphism (SSCP). After parallel detection of peripheral blood (PB) and bone marrow (BM) samples from 15 patients with BM and 11 patients with PB, the expected PCR-specific PCR products ranged from 80 to 110 bp, of which 9 obtained the same PCR products as PB and 8 For example, there is one amplified band, SSCP analysis shows two single strands, and the other one PCR product is two amplification strands. SSCP analysis shows three single strands. In the same patient, PB and BM specimens were single-stranded Moving speed consistent. It is suggested that at least 60% of PB patients with PB have the same PCR products and consistent single-stranded conformation. The results confirmed that peripheral blood B cells are involved in the pathogenesis of MM.