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目的:应用核-壳色谱柱优化人参须药材中人参皂苷Rg1、人参皂苷Re、及人参皂苷Rb1的高效液相色谱含量测定方法。方法:采用Kinetex核-壳色谱柱(4.6 mm×150 mm,2.6μm);流动相:乙腈(A)-水(B)梯度洗脱(0～6 min:19%A;6～13 min:19%～29%A;13～18 min:29%～40%A;18～25 min:40%～19%A);流速为1.8mL/min,检测波长203 nm,柱温30℃,进样量10μL。结果:人参皂苷Rg1、人参皂苷Re及人参皂苷Rb1的线性范围分别为0.4242～3.3936μg(r1=0.9992)、0.6504～5.2032μg(r2=0.9992)和1.3442～10.7536μg(r3=0.9992),平均加样回收率分别为99.91%(RSD=0.9%)、100.59%(RSD=1.6%)和102.64%(RSD=1.8%)。结论:该测定方法简便可行,准确可靠,重现性好,结果稳定,可用于人参及参须的含量测定。
OBJECTIVE: To determine the content of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in ginseng medicinal herbs by high performance liquid chromatography with core-shell chromatography. METHODS: Kinetex core-shell columns (4.6 mm × 150 mm, 2.6 μm) were used. The mobile phase consisted of gradient elution with acetonitrile (A) and water (0-6 min: 19% A; 19% -29% A; 13-18 min: 29-40% A; 18-25 min: 40-19% A); the flow rate was 1.8 mL / min; the detection wavelength was 203 nm; Sample size 10μL. Results: The linear ranges of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 0.4242 ~ 3.3936μg (r1 = 0.9992), 0.6504 ~ 5.2032μg (r2 = 0.9992) and 1.3442 ~ 10.7536μg (r3 = 0.9992) The recovery rates were 99.91% (RSD = 0.9%), 100.59% (RSD = 1.6%) and 102.64% (RSD = 1.8%), respectively. Conclusion: The method is simple and feasible, accurate and reliable, reproducible, and stable. It can be used for the determination of ginseng and ginseng.