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目的分析辽阳市2011年-2013年各类艾滋病初筛实验室送检的HIV抗体筛查阳性样品的确证符合率,找出相应的影响因素,为今后市内各艾滋病初筛实验室提高HIV抗体检测工作质量提供指导。方法对送检的HIV抗体筛查阳性样本,采用免疫印迹(WB)进行确认分析。结果 2011年-2013年辽阳市艾滋病确认实验室共收到HIV抗体筛查阳性样本128例,85例确证阳性,阳性率为66.41%。2011年-2013年的确证阳性率分别为67.86%、67.50%、65.00%。3类HIV抗体初筛实验室中,疾病预防控制机构初筛实验室HIV确证阳性率为82.14%,医疗机构筛查实验室为60.71%,采供血机构筛查实验室为31.25%。结论全市2011年-2013年每年确证阳性率变化不大,需要加强HIV初筛实验室的检测水平,加强人员培训,规范实验室的仪器、设备,严格按照《全国艾滋病检测技术规范》(2009年修订版)的要求开展HIV检测工作。
Objective To analyze the exact coincidence rate of positive HIV antibody screening samples from various AIDS screening laboratories in Liaoyang from 2011 to 2013 and find out the corresponding influencing factors so as to enhance the HIV antibody screening in various AIDS screening laboratories in the future Test the quality of work to provide guidance. Methods Positive samples were screened for HIV antibodies tested and confirmed by Western blotting (WB). Results From 2011 to 2013, a total of 128 positive HIV antibody screening samples were obtained from HIV / AIDS confirmed laboratories in Liaoyang. 85 positive samples were confirmed positive, with a positive rate of 66.41%. The positive rates in 2011-2013 were 67.86%, 67.50% and 65.00% respectively. Among the three types of HIV antibody screening laboratories, the positive rate of HIV confirmatory screening by the CDC was 82.14%, that of the screening laboratories of medical institutions was 60.71%, and that of blood screening laboratories was 31.25%. Conclusion The positive rate of positive confirmation in the whole city from 2011 to 2013 has little change. It is necessary to strengthen the testing level of HIV screening laboratories, strengthen personnel training, standardize laboratory equipment and equipment, strictly follow the “National AIDS Testing Technical Specifications” (2009 Revised version) requirements for HIV testing.