目的:研究宁夏枸杞总黄酮对人恶性胶质瘤U87-MG细胞增殖、迁移以及侵袭的影响。方法:采用贴壁法培养人恶性胶质瘤细胞。实验分为空白对照组(不加任何处理)和黄酮处理组(20,40,80,160 mg·L-1)。用CCK-8法测定不同浓度宁夏枸杞总黄酮作用U87-MG细胞不同时间后,细胞的增殖变化情况;RT-PCR和real-time PCR法检测肿瘤细胞内增殖细胞核抗原(PCNA)mRNA水平的表达;免疫细胞化学(immunocytochemistry,ICC)检测PCNA蛋白表达;Transwell和细胞划痕实验检测细胞的迁移能力;Transwell法检测细胞侵袭能力。结果:与对照组相比,处理组的U87-MG细胞增殖被抑制,且呈一定的时间-剂量依赖关系;处理组的PCNA mRNA和蛋白水平含量均降低,迁移以及侵袭能力减弱;且上述指标的变化随枸杞总黄酮浓度的增加而趋于明显。结论:宁夏枸杞总黄酮能够抑制恶性胶质瘤细胞的增殖、迁移以及侵袭。 Objective: To study the effects of Lycium barbarum total flavone on the proliferation, migration and invasion of human malignant glioma U87-MG cells. Methods: Human malignant glioma cells were cultured by adherent method. The experiment was divided into blank control group (without any treatment) and flavonoid treatment group (20,40,80,160 mg · L-1). The CCK-8 method was used to determine the proliferation of U87-MG cells after different concentrations of total flavonoids of Lycium barbarum L. The expression of proliferating cell nuclear antigen (PCNA) mRNA was detected by RT-PCR and real-time PCR Immunocytochemistry (ICC) was used to detect the expression of PCNA protein. Transwell and cell scratch assay were used to detect the migration of cells. Transwell assay was used to detect cell invasion. Results: Compared with the control group, the proliferation of U87-MG cells was inhibited in a dose-dependent manner in a time-dependent manner. The content of PCNA mRNA and protein in the treated group decreased, and the migration and invasive ability of U87-MG cells decreased. Changes with the total flavonoids concentration of Lycium tend to be obvious. Conclusion: The total flavonoids of Lycium barbarum can inhibit the proliferation, migration and invasion of malignant glioma cells.