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生物化学教学实验室在学生自选实验的基础上 ,探索用分光光度法对人体运动前后尿中乳酸含量进行测定。实验得出静止时人体乳酸含量约为 0 .3mmol/ L,剧烈运动后提高 5 0～ 30 0倍的结论 ,具体提高倍数视体个体和运动程度而有所差异 ,运动后休息 2 h左右尿乳酸含量可恢复正常。该实验在本科生教学实验中试做 ,效果良好 ,有利于加深同学们对 Cori循环的理解。
On the basis of students’ optional experiments, the teaching laboratory of biochemistry explores the determination of lactic acid in urine before and after exercise by spectrophotometry. The experimental results show that the human lactic acid content is about 0.3mmol / L at rest and increased 50 ~ 300 times after vigorous exercise. The results show that there are differences between the subjects with multiple ocular bodies and the degree of exercise, Lactic acid content can return to normal. The experiment in undergraduate teaching experiment try out, the effect is good, help to deepen the students understanding of the Cori cycle.