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目的:建立并优化高效液相色谱(HPLC)法对决明子药材中6种蒽醌化合物的分析方法,对市售决明子(包括伪品)进行含量分析,为决明子的质量控制提供方法。方法:采用HPLC法对决明子中的橙黄决明素、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进行分析。比较了不同产地、不同品种、不同贮藏时间决明子中蒽醌含量的差异。结果:钝叶决明、小决明、茳芒决明蒽醌的含量差异明显,不同产地决明子(钝叶决明)在橙黄决明素、大黄素、大黄酚、大黄素甲醚的含量上存在差异,其中大黄酚的含量差异较大。其中符合《中国药典》2015年版一部决明子项对于大黄酚的含量要求的只有50%;不同贮藏时间是大黄酚含量差异较大的原因。结论:该方法准确、简便、重现性好,可作为决明子质量控制的方法;决明子采收和产地加工对于决明子大黄酚的含量至关重要。
OBJECTIVE: To establish and optimize a method for the determination of six anthraquinone compounds in Cassia seed by high performance liquid chromatography (HPLC), analyze the contents of cassia seeds (including counterfeit) in commercial cassia seeds, and provide a method for the quality control of cassia seed. Methods: HPLC analysis of Cassia toxins Cassia, aloe-emodin, rhein, emodin, chrysophanol, emodin methyl ether were analyzed. The differences of anthraquinone content in cassia seed from different producing areas, different varieties and different storage time were compared. Results: The contents of Anthraquinone in the leaves of Cassia obtusifolia, Cassia toxis and Cassia yunnanensis were significantly different. The content of anthocyanin in Cassia obtusifolia There are differences, including large differences in the content of chrysophanol. Which meet the “Chinese Pharmacopoeia” 2015 edition of a Cassia sub-items for chrysophanol content requirements of only 50%; different storage time is chrysophanol content greater differences. Conclusion: The method is accurate, simple and reproducible. It can be used as a quality control method for cassia seed; cassia seed harvesting and processing of origin is crucial for the content of cassia seed chrysophanol.