论文部分内容阅读
Pm21具有强抗白粉病特性,位于簇毛麦6V染色体短臂(6VS)上。染色体分析和白粉病抗性检测表明,Pm21在受体小麦内是稳定遗传的。筛选与Pm21相连锁分子标记,在小麦抗白粉病辅助选择育种上有重要意义。利用RAPD和AFLP分子标记方法,对簇毛麦6V染色体和含有6V的小麦-簇毛麦代换系、6VS的易位系6AL/6VS、6DL/6VS进行分子标记筛选,以分析位于6VS上的分子标记及其与Pm21的关系。RAPD检测表明,OPK08910特异片段存在于含簇毛麦6V染色体的代换系(6A/6V)、易位系(6AL/6VS,6DL/6VS)和簇毛麦中。AFLP检测显示,PstⅠ+AGG/MseⅠ+CAC、PstⅠ+ACC/MseⅠ+CCT和PstⅠ+AAG/MseⅠ+CGC共3对引物分别可以在6A/6V、6AL/6VS、6DL/6VS和VV中扩增出264bp、218bp和232bp特异带,并与抗白粉病基因Pm21共分离,因此,上述来源于6VS上的4个新的分子标记,可作为源自簇毛麦Pm21基因的选择标记,用于小麦抗病育种。
Pm21 has strong resistance to powdery mildew and is located on 6VS of 6VS. Chromosome analysis and powdery mildew resistance assays showed that Pm21 was stably inherited within recipient wheat. Screening and Pm21 linked molecular markers, wheat powdery mildew assisted selection breeding is important. The RAPD and AFLP molecular markers were used to screen the 6V chromosomes of 6-sowed wheat and 6V wheat-cluster-wool combinations, 6AL / 6VS and 6DL / 6VS of 6VS, Molecular Markers and Their Relationship with Pm21. RAPD analysis showed that the specific fragment of OPK08910 was found in 6A / 6V, 6AL / 6VS, 6DL / 6VS and cluster hay plants of 6V chromosomes of H. villosa. AFLP analysis showed that 3 pairs of primers of PstⅠ + AGG / MseⅠ + CAC, PstⅠ + ACC / MseⅠ + CCT and PstⅠ + AAG / MseⅠ + CGC could amplify in 6A / 6V, 6AL / 6VS, 6DL / 6VS and VV Out of 264bp, 218bp and 232bp specific bands and was co-segregated with the powdery mildew resistance gene Pm21. Therefore, the above four new molecular markers derived from 6VS can be used as selection markers for the Pm21 gene derived from Hamster, for wheat Disease-resistant breeding.