生物组织细胞的玻璃化冷冻保存

来源 :中国临床康复 | 被引量 : 0次 | 上传用户:awii0813
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Vitrificated indicates the high adhesion state of solution at extremely low temperature(-115℃ )[1].No ice crystals are formed when extracellular and intracellular solution is under state of vitrification,and hence eliminating mechanic injury and preserving activity of biological tissues.In 1980s, cryopreservation of mouse embrgos, red cells,rats ocytes,human monocytes was successfully performed[2-4].After that,some researchers successfully preserved cea and skin by vitrification[5-8].In order to elucidate preservation of cells and tissues by vitrification and widen apply of vitrification,we investigate principle of vitrification, solution,devitrification and its application.
其他文献
It has been thoroughly investigated that the collagenase can dissolve the intervertebral disc,therefore the suggestion of introduction of collagenase to the adj
Background: It is significant how to reserve residual myodynamia of patients with spinal cord injury, increase the ability of self care and make them return to
Background:Sodium β escin is involved in Aesculus chinensis Bunge, could increase the tension of veins,improve blood rheology making the blood flow faster,fast
Objective To observe effects of meshed relaxing short incisions (MRSI) on the content of endothelin (ET) in rat tension skin flap,and to investigate the mechani
Objective To study changes and significance of endothelin(ET) in rat cerebral concussion.Methods 80 Wistar male rats were used for animal model of cerebral conc
Persistent vegetative state (PVS) is one special kind of disturbance of consciousness.At present, there is no effective therapeutic method for PVS,and most pati
Background: Cicatricial deformation in child burn of hand often leads to severe squaelae. According to different causes of disease , different plastic surgery w
1 Subjects and method rn1.1 Choice of cases All cases should match the following condition.⑴ The patients were admitted in 6 h after trauma and diagnosed origi
Objective To study on the CDKN2/P16 gene in primary osteosarcoma.Method By using molecular biological methods that inclued genome DNA extraction from paraffined
Objective To observe the effect of physical factors and promotion canalization on closed injury of brain.Method 68 cases of closed injury of brain were given th