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目的:建立大血藤药材中绿原酸的含量测定方法。方法:采用高效液相色谱法,Diamonsil ODS-C18色谱柱(4.6mm×250 mm,5μm),流动相乙腈-4%磷酸水(11∶89),流速1.0 mL.min-1,检测波长327 nm,柱温25℃。结果:绿原酸在0.000 102～0.010 2 mg线性关系良好。加样回收率为102.4%,RSD 1.7%(n=6)。结论:采用HPLC测定大血藤药材中绿原酸的含量,样品处理方法便捷,测定方法简单,结果准确可靠,可用于大血藤饮片、大血藤制剂的质量控制。
Objective: To establish a method for determination of chlorogenic acid in Daoxuentang herbs. Methods: High performance liquid chromatography (HPLC) was used on a Diamonsil ODS-C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase of acetonitrile-4% phosphoric acid (11:89) at a flow rate of 1.0 mL · min- nm, column temperature 25 ℃. Results: The chlorogenic acid had a good linearity at 0.000 102 ~ 0.010 2 mg. The recovery rate was 102.4% with RSD 1.7% (n = 6). Conclusion: The determination of chlorogenic acid in Rhodiola rosea L. by HPLC is simple and convenient, the method is simple and the method is simple and accurate. The method can be used for the quality control of Da Xue Ban decoction and Rhodiola sachalinensis.