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目的:探讨大鼠脑出血应激状态下对肠道黏膜屏障功能的影响。方法:将30只健康成年雄性SD大鼠随机分为脑出血组与对照组,脑出血组采用Nath改良法注射自体动脉血建立大鼠实验性脑出血模型,对照组实验步骤同脑出血组,但不注射自体动脉血,两组大鼠术后按1、3、6、12、24 h分为5个亚组,各亚组大鼠3只。通过测定各亚组大鼠血浆D-乳酸的浓度和小肠二胺氧化酶(DAO)活性来判定脑出血对肠道黏膜屏障功能的影响。取距回盲部5~8 cm小肠组织,制成光镜标本,观察肠绒毛结构的变化,采用四级评分系统(0~3分)对小肠损伤情况进行判定。结果:光镜下观察脑出血组大鼠第3、6、12小时的小肠黏膜屏障损伤评分明显高于对照组(P<0.05),脑出血后1 h血浆D-乳酸水平即开始升高,第1、3、6、12小时均高于对照组,两组比较差异有统计学意义(P<0.05),至24 h后下降至略高于对照组(P>0.05);脑出血组大鼠小肠DAO活性明显下降,随时间变化持续下降,第6、12、24小时低于对照组,两组比较差异有统计学意义(P<0.05)。结论:大鼠脑出血应激状态下可导致小肠肠道黏膜屏障损害。
Objective: To investigate the effect of rat brain hemorrhage under stress on intestinal mucosal barrier function. Methods: Thirty healthy adult male Sprague-Dawley rats were randomly divided into intracerebral hemorrhage group and control group. Experimental intracerebral hemorrhage model was established by injecting autologous arterial blood with Nath modified method in cerebral hemorrhage group. But did not inject autologous arterial blood. The two groups of rats were divided into 5 subgroups at 1, 3, 6, 12 and 24 hours after operation, and 3 rats in each subgroup. The effects of intracerebral hemorrhage on the intestinal mucosal barrier function were determined by measuring the concentration of plasma D-lactate and the activity of diamine oxidase (DAO) in each subgroup of rats. The small intestine tissue of 5-8 cm away from the ileocecal part was made into light microscope to observe the change of intestinal villus structure. The severity of small intestine injury was evaluated by a four-grade scoring system (0-3 points). Results: The damage score of intestinal mucosal barrier in 3h, 6h and 12h after intracerebral hemorrhage was significantly higher than that in control group (P <0.05). The plasma D-lactic acid level began to increase at 1 hour after intracerebral hemorrhage, 1, 3, 6, 12 hours were higher than the control group, the difference between the two groups was statistically significant (P <0.05), after 24 hours decreased slightly higher than the control group (P> 0.05); cerebral hemorrhage group The activity of DAO in the small intestine of rats decreased significantly, and the changes of DAO kept decreasing with the passage of time. The 6th, 12th and 24th hours were lower than the control group. There was significant difference between the two groups (P <0.05). Conclusion: Under the condition of cerebral hemorrhage in rats, intestinal mucosal barrier damage can occur.