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为观察大鼠缺锌的病理表现，用ＥＤＴＡ螯合的方法，去除了饲料成分中的绝大部分锌，配制成缺锌饲料（含锌１．３６μｇ／ｇ饲料），并制作正常含锌饲料（３１．０６μｇ／ｇ饲料）、高锌饲料（７３．３９μｇ／ｇ饲料）。ＳＤ大鼠随机分成３组：缺锌饲料（Ｌ）组，正常含锌饲料（Ｎ）组，高锌饲料（Ｈ）组。结果发现，Ｌ组出现进食减少，生长停滞，体重下降，Ｎ、Ｈ组大鼠体重增长。Ｌ组大鼠血清锌、骨锌、碱性磷酸酶（ＡＫＰ）明显低于Ｈ、Ｎ组，出现异食癖、稀便、精神萎靡、肢体僵硬、毛发稀疏等缺锌症状表现。表明本研究制作的缺锌模型是成功的，其关键是去除饲料成分中的锌。
In order to observe the pathological manifestation of zinc deficiency in rats, EDTA-chelating method was used to remove most of the zinc in the feed ingredients to make zinc-deficient feed (containing 1.36 μg / g zinc) and to make normal zinc-containing feed (31.06 μg / g feed), high zinc feed (73.39 μg / g feed). SD rats were randomly divided into three groups: zinc-deficient diet (L) group, normal zinc-containing diet (N) group, high zinc diet (H) group. The results showed that, L group decreased food intake, growth arrest, weight loss, N, H group rats weight gain. Serum Zinc, Bone Zinc, and Alkaline Phosphatase (AKP) of rats in L group were significantly lower than those in H and N groups, indicating the presence of zinc deficiency symptoms such as pica, loose stool, apathetic, limb stiffness and sparse hair. It shows that the zinc deficiency model produced in this study is successful, and the key point is to remove the zinc from the feed ingredients.