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建立测定人血浆中倍他米松的LC-MS/MS方法。采用Venusil XBP C8(200 mm×3.9 mm ID,5μm)色谱柱,流动相为甲醇-水(含甲酸铵5 mmol.L-1)(80∶20),流速0.4 mL.min-1;质谱仪离子源为电喷雾离子源(ESI),正离子模式检测,监测离子为393.3→355.2(倍他米松)和361.3→343.2(泼尼松龙,内标)。血浆样本用乙酸乙酯处理。倍他米松在0.5~80.0 ng.mL-1线性关系良好(r=0.999 2),血浆低、中、高3种浓度(1.0,10.0,60.0ng.mL-1)平均提取回收率为88.24%,定量限为0.5 ng.mL-1。本方法操作简便、准确、灵敏,适用于复方倍他米松注射液人体药代动力学研究。
To establish a method for the determination of betamethasone in human plasma by LC-MS / MS. A venusil XBP C8 (200 mm × 3.9 mm ID, 5 μm) column was used with a mobile phase of methanol-water (5 mmol·L-1) (80:20) and a flow rate of 0.4 mL · min- The ion source was electrospray ion source (ESI) with positive ion mode detection. The monitored ions were 393.3 → 355.2 (betamethasone) and 361.3 → 343.2 (prednisolone, internal standard). Plasma samples were treated with ethyl acetate. The average extraction recoveries of betamethasone at 0.5 ~ 80.0 ng.mL-1 (r = 0.999 2), plasma low, medium and high at concentrations of 1.0, 10.0 and 60.0 ng.mL-1 were 88.24% , The limit of quantification was 0.5 ng.mL-1. The method is simple, accurate and sensitive and is suitable for the study of human pharmacokinetics of compound betamethasone injections.