论文部分内容阅读
常用中药牡丹和芍药种子中含有茋类成分,具有多种药理活性,为了开发和利用牡丹种子资源,该文选择种子中含量相对较高的10种茋类成分suffruticosol A,suffruticosol B,suffruticosol C,trans-resveratrol,cis-ε-viniferin,trans-ε-viniferin,cissuffruticosol D,cis-gnetin H,trans-suffruticosol D和trans-gnetin H为指标进行含量测定。采用HPLC-DAD,YMC-pack ODS-A色谱柱(4.6 mm×250 mm,5μm);流动相以甲醇-水梯度洗脱,流速为1.0 m L·min-1;柱温25℃;检测波长为230 nm。同时采用清除DPPH自由基的方法初步评价其抗氧化作用。结果表明:所建立的方法能够同时测定10种茋类成分的含量;牡丹和芍药种子中的茋类成分主要集中于种皮部位(总质量分数达16.7%以上),种仁中含量很低(总质量分数不到0.3%),种皮提取物富含茋类成分,总含量达75%以上。种皮提取物及10种茋类成分多具有较好的抗氧化潜力。该文对牡丹和芍药资源的综合利用,特别是种皮资源的有效开发和利用奠定了基础。
Common Peony and Paeonia lactiflora seeds contain many kinds of pharmacological activities. In order to develop and utilize peony seed resources, ten kinds of Succulents, such as suffruticosol A, suffruticosol B, and suffruticosol C, trans-resveratrol, cis-ε-viniferin, trans-ε-viniferin, cissuffruticosol D, cis-gnetin H, trans-suffruticosol D and trans-gnetin H were determined. The mobile phase was eluted with a methanol-water gradient at a flow rate of 1.0 mL · min-1 with a column temperature of 25 ° C using a YMC-pack ODS-A column (HPLC-DAD, 4.6 mm × 250 mm, 5 μm) 230 nm. At the same time, the DPPH radical scavenging method was used to evaluate its antioxidant activity. The results showed that the established method could simultaneously determine the contents of 10 species of Scutellaria species. The contents of Scutellariae in Peony and Paeonia lactiflora mainly concentrated on the seed coat (the total mass fraction was over 16.7%) and the content of seeds was very low The total mass fraction of less than 0.3%), seed coat extract is rich in clams, the total content of more than 75%. Seed coat extract and 10 kinds of 茋 class ingredients have more good antioxidant potential. This article lays the foundation for the comprehensive utilization of peony and peony resources, especially the effective exploitation and utilization of seed coat resources.