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目的分析研讨化学发光免疫测定在梅毒螺旋体抗体检测中的临床价值。方法收集127例梅毒患者以及98例非梅毒病人的血清,分别应用化学发光免疫测定法、TPPA法、RPR法对血清中梅毒螺旋体抗体进行监测,将检测结果纳入对比研讨中。结果组间和组内在不同梅毒螺旋体抗体浓度下CV分别为6.81%、8.29%、8.48%和6.71%、8.43%、8.51%,皆小于10%。并且CLIA分析仪灵敏度比TPPA高,组间数据有统计学意义(P<0.05);通过CLIA分析仪对梅毒螺旋体抗体进行检测,干扰物加入前后所测量得出的值差异为3.19%(吸光度/临界值);CLIA法与TPPA法所检测的阳性率对比无明显差异(P>0.05);但两种检测结果均比RPR法要高,组间数据有统计学意义(P<0.05)。结论化学发光免疫测定法检测梅毒螺旋体抗体的结果准确率较高,且灵敏度较高,不容易受到诸多外在因素干扰及限制,值得临床应用并推广。
Objective To study the clinical value of chemiluminescence immunoassay in the detection of Treponema pallidum antibody. Methods Serum samples from 127 patients with syphilis and 98 patients with non-syphilis were collected and analyzed by chemiluminescence immunoassay, TPPA method and RPR method, respectively. The results were included in the comparative study. Results The CVs were 6.81%, 8.29%, 8.48% and 6.71%, 8.43% and 8.51%, respectively, within the groups and within the group, all less than 10%. The sensitivity of CLIA was higher than that of TPPA (P <0.05). The detection of Treponema pallidum antibody by CLIA was 3.19% before and after interference. (Absorbance / (P> 0.05). However, there was no significant difference between the positive rate of CLIA assay and that of TPPA assay (P> 0.05). Conclusion Chemiluminescence immunoassay for the detection of Treponema pallidum antibody results with high accuracy and high sensitivity, not easy to be disturbed by many external factors and limitations, it is worthy of clinical application and promotion.