论文部分内容阅读
本文以两个大豆品种为材料,研究了利用茎尖外植体进行离体快繁的可行性.结果表明:以MSB为基本培养基,附加不同浓度的NAA和KT均能促进侧芽增殖.其中,以NAA0.2mg/L、K T0.5mg/L效果最好。侧芽切割后接种于1/2 MS附加NAA0.1mg/L的培养基上可诱导生根,完整植株移栽后极易成活.
In this paper, two soybean cultivars were used as materials to study the feasibility of rapid propagation in vitro using shoot tip explants.The results showed that the addition of different concentrations of NAA and KT could promote the proliferation of lateral shoots with MSB as the basic medium , With NAA0.2mg / L, K T0.5mg / L best. The lateral shoots were inoculated on 1/2 MS supplemented NAA0.1mg / L medium can induce rooting, the whole plant is easy to survive after transplanting.