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目的:研究Clusterin和p21基因在膀胱癌中的表达和临床意义及二者的相关性。方法:采用免疫组织化学检测20例正常膀胱组织(NB)、60例膀胱癌组织(BTCC)中Clusterin和p21蛋白的表达情况,根据染色强度和阳性细胞数作半定量分析。结果:Clus-terin、p21蛋白在膀胱癌中的阳性表达率明显高于正常膀胱组织,阳性表达率分别为70.0%、63.3%;20.0%、15.0%(P<0.05);二者在低分化、浸润性肿瘤的阳性表达率明显高于高分化、浅表性肿瘤(P<0.05),Clusterin和p21在膀胱癌中的表达存在正性相关(P<0.05)。结论:Clusterin、p21蛋白的表达与膀胱癌病理组织学分级、临床分期相关,二者联合检测可为膀胱癌的诊断及预后提供一定参考价值。
Objective: To study the expression and clinical significance of Clusterin and p21 in bladder cancer and their relationship. Methods: The expressions of Clusterin and p21 protein in 20 cases of normal bladder tissue (BTCC) and 60 cases of bladder cancer (BTCC) were detected by immunohistochemistry. Semiquantitative analysis was made based on the staining intensity and the number of positive cells. Results: The positive rates of Clus-terin and p21 protein in bladder cancer were significantly higher than those in normal bladder tissues (70.0%, 63.3%, 20.0%, 15.0%, respectively) . The positive rate of invasive tumor was significantly higher than that of well differentiated and superficial tumors (P <0.05). There was a positive correlation between the expression of Clusterin and p21 in bladder cancer (P <0.05). Conclusion: The expression of Clusterin and p21 protein is correlated with the histopathological grade and clinical stage of bladder cancer. The combined detection of Clusterin and p21 protein may provide some reference value for the diagnosis and prognosis of bladder cancer.