作者研究了黑茶藨Ribes nigrum L.种子中的几种粗多糖和精制多糖对幽门螺杆菌粘附的抑制作用。用水提醇沉方法,从该植物种子中提取粗多糖(RPS)。RPS在DEAE-Sephacel柱(pH8.0)上,用离子交换色谱法分离出2个次级部位,即用水洗脱的部位和用磷酸钠溶液(0.1mol/L)洗脱的部位。将异硫腈酸荧光素(FITC)标记的幽门螺杆菌G27株悬液分 The authors studied the inhibitory effects of several crude polysaccharides and refined polysaccharides on the adhesion of Helicobacter pylori in seeds of the black tea aphid Ribes nigrum L. The water extraction and alcohol precipitation method was used to extract the crude polysaccharide (RPS) from the seeds of the plant. The RPS was separated on a DEAE-Sephacel column (pH 8.0) by ion exchange chromatography and the two secondary sites were eluted with water and eluted with sodium phosphate solution (0.1 mol/L). Suspension of fluorescein isothiocyanate (FITC)-labeled H. pylori strain G27