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建立了水样中甲氨基阿维菌素苯甲酸盐(甲维盐)残留量的柱前衍生高效液相色谱测定方法.水样经乙酸乙酯提取后,运用N-甲基咪唑-乙腈(NMIM-CAN)(1+1)、三氟乙酸酐-乙腈(TFAA-CAN)(1+1)在避光条件下进行柱前衍生化反应,反应20min后,用高效液相色谱-荧光检测器进行测定.结果表明,水中甲维盐添加浓度在0.0001—0.01mg.l-1范围,方法的回收率为78.4%—115.2%,变异系数为3.0%—11.3%,方法的最低定量限为0.0036mg.l-1,方法的灵敏度、精密度和准确度完全满足农药残留分析的要求.甲维盐在巢湖水中的残留量低于该方法的检出限,稻田水中的残留量为0.54mg.l-1.
A method for the determination of emamectin benzoate (carbaryl salt) in water samples was established by pre-column derivatization with high performance liquid chromatography.The aqueous sample was extracted with ethyl acetate and treated with N-methylimidazole-acetonitrile (1 + 1), trifluoroacetic anhydride-acetonitrile (TFAA-CAN) (1 + 1) under the condition of dark. After the reaction for 20min, Detector.The results showed that the recovery rate of the method was 0.0001-0.01mg.l-1, the recovery rate was 78.4% -115.2%, the coefficient of variation was 3.0% -11.3%, and the lowest limit of quantification Is 0.0036mg.l-1, the sensitivity, precision and accuracy of the method fully meet the requirements of pesticide residue analysis.Marvin residual in Chaohu Lake water is lower than the detection limit of the method, the residual amount of paddy water is 0.54 mg.l-1.