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用免疫荧光、激光共聚焦显微镜图像分析及膜片钳等技术研究了鼻咽癌上皮cNE-2Z细胞容积激活性氯通道候选基因C1C-3的表达及其在细胞周期中与容积激活性氯电流及细胞容积调节性回缩(regulatorly volume decrease,RVD)的关系。结果显示,CNE-2Z细胞表达CIC-3。C1C-3蛋白主要位于细胞内而不是在细胞膜上,其表达水平及其在细胞中的分布呈细胞周期依赖性。G1期细胞的C1C-3表达水平较低而S期则较高,M期细胞的表达水平中等。在细胞周期中,C1C-3表达水平与细胞RVD能力及容积激活性氯电流水平呈反比。上述观察结果提示,C1C-3可能参与细胞周期的调节,但CNE-2Z细胞中的C1C-3可能不是与RVD有关的氯通道。
Immunofluorescence, confocal laser scanning microscopy image analysis and patch-clamp technique were used to investigate the expression of C1C-3, a candidate gene of chloride channel in nasopharyngeal epithelial cell line cNE-2Z, and its relationship with volume-activated chloride current And regulator of volume decrease (RVD). The results show that CNE-2Z cells express CIC-3. The C1C-3 protein is mainly located in the cell but not in the cell membrane, and its expression level and its distribution in the cell are in a cell cycle-dependent manner. The expression of C1C-3 in G1 phase was lower than that in S phase, while it was moderate in M phase. In the cell cycle, C1C-3 expression levels and cell RVD capacity and volume-activated chloride current levels are inversely proportional. The above observations suggest that C1C-3 may be involved in cell cycle regulation, but C1C-3 in CNE-2Z cells may not be the RVD-related chloride channel.