在参考并改进其他组织中羟脯氨酸（Ｈｙｐ）测定方法的基础上，对大鼠肺组织Ｈｙｐ测定方法的实验条件进行了研究。结果表明，①样品酸水解时间为 ２～３ ｈ；②测量前无需调 ｐＨ；③显色反应的温度和时间分别为 ８０ ℃和 １０ ｍｉｎ；④显色后无需冰水冷却。该方法重复性好，回收率高，在放射性肺炎研究中应用时，观察到肺组织Ｈｙｐ含量随照后时间的动态变化及补硒保护均有明显规律。 On the basis of referring to and improving the determination of hydroxyproline (Hyp) in other tissues, the experimental conditions of Hyp determination in rat lung tissue were studied. The results showed that: ① acid hydrolysis time of 2 ~ 3 h; ② no need to adjust pH before measurement; ③ color reaction temperature and time were 80 ℃ and 10 min; ④ no ice water cooling after color. The method has good repeatability and high recovery rate. When applied in the research of radiation pneumonitis, the dynamic changes of Hyp content in lung tissue and the protection of selenium are obviously observed.