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目的观察异甘草素对大鼠脑胶质瘤组织p53和血管内皮生长因子(VEGF)表达的影响,探讨异甘草素抗胶质瘤作用机制。方法 36只清洁级Wistar雌性大鼠随机分为2组:假手术组(n=12)与模型组(n=24)。建立大鼠C6胶质瘤模型,第8天将模型组再细分为模型组与异甘草素组。异甘草素组腹腔注射异甘草素(500μg·kg-1·d-1),模型组腹腔注射等量生理盐水,连续给药一周后处死大鼠。免疫组织化学方法和Western blot方法检测肿瘤组织p53和VEGF蛋白表达水平的变化。结果免疫组织化学结果和Western blot结果显示,与假手术组相比,模型组与异甘草素组大鼠脑组织p53和VEGF的蛋白表达的平均光密度值显著增加,差异有统计学意义(P<0.01)。两两比较,异甘草素组大鼠脑组织p53和VEGF的蛋白表达的平均光密度值显著低于模型组,差异有统计学意义(P<0.01)。结论异甘草素能够降低脑胶质瘤大鼠p53和VEGF的表达,这可能是其治疗胶质瘤的作用机制之一。
Objective To investigate the effect of isoliquiritigenin on the expression of p53 and vascular endothelial growth factor (VEGF) in rat glioma and to explore the mechanism of action of isoliquiritigenin against glioma. Methods Thirty - six clean Wistar female rats were randomly divided into 2 groups: sham operation group (n = 12) and model group (n = 24). The rat C6 glioma model was established. On the 8th day, the model group was subdivided into model group and isoliquiritigenin group. Isoliquiritigenin was intraperitoneally injected with isoliquiritigenin (500μg · kg-1 · d-1), and the model group was injected intraperitoneally with the same amount of saline. Rats were sacrificed one week after continuous administration. Immunohistochemistry and Western blot were used to detect the expression of p53 and VEGF in tumor tissue. Results The results of immunohistochemistry and Western blot showed that the average optical density of protein expression of p53 and VEGF in model group and isoliquiritigenin group were significantly increased compared with sham-operation group, the difference was statistically significant (P <0.01). The pairwise comparison showed that the average optical density of protein expression of p53 and VEGF in isoliquiritigenin group was significantly lower than that in model group (P <0.01). Conclusion Isoliquiritigenin can reduce the expression of p53 and VEGF in glioma rats, which may be one of the mechanisms of glioma treatment.