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目的:探讨大黄素抑制体内外胰腺癌转移的作用及其机制。方法:不同浓度大黄素(10,20,40μmol.L-1)作用人胰腺癌细胞株SW1990 2 h后,观察大黄素对SW1990细胞迁移和侵袭的作用;不同浓度大黄素(10,20,40μmol.L-1)作用SW1990细胞48 h后,Western blot印迹检测胰腺癌细胞中NF-κB和MMP-9表达的变化;建立胰腺癌原位移植模型,分成对照组、大黄素低、高剂量组(20,40 mg.kg-1),实验结束后观察大黄素对裸鼠胰腺癌转移的抑制作用;免疫组织化学法检测裸鼠胰腺肿瘤组织中CD34,NF-κB,MMP-9的阳性表达。结果:大黄素可抑制体外人胰腺癌SW1990细胞迁移和侵袭,呈浓度依赖性;大黄素可下调人胰腺癌SW1990细胞中NF-κB和MMP-9的表达水平;与对照组相比较,大黄素低、高剂量均可明显抑制瘤荷裸鼠中胰腺癌转移,并下调CD34,NF-κB和MMP-9在胰腺肿瘤组织中的阳性表达(P<0.05)。结论:大黄素可抑制体内外胰腺癌转移,该作用可能通过下调NF-κB和MMP-9表达而实现。
Objective: To investigate the effect of emodin on pancreatic cancer metastasis in vitro and in vivo and its mechanism. Methods: The human pancreatic cancer cell line SW1990 was treated with different concentrations of emodin (10, 20, 40 μmol·L-1) for 2 hours to observe the effect of emodin on the migration and invasion of SW1990 cells. The effects of different concentrations of emodin (10, 20 and 40 μmol The expression of NF-κB and MMP-9 in pancreatic cancer cells was detected by Western blotting after SW1990 cells were treated with L-1 for 48 hours. The pancreatic cancer orthotopic transplantation model was established and divided into control group, low and high emodin group (20,40 mg.kg-1). After the experiment, the inhibitory effect of emodin on the metastasis of pancreatic cancer in nude mice was observed. The positive expression of CD34, NF-κB and MMP-9 in pancreatic tumor tissues were detected by immunohistochemistry . Results: Emodin could inhibit the migration and invasion of SW1990 cells in vitro in a concentration-dependent manner. Emodin could down-regulate the expression of NF-κB and MMP-9 in human pancreatic cancer SW1990 cells. Compared with the control group, Both low and high doses could significantly inhibit the metastasis of pancreatic cancer in nude mice and down-regulate the expression of CD34, NF-κB and MMP-9 in pancreatic tumor (P <0.05). Conclusion: Emodin can inhibit the metastasis of pancreatic cancer in vitro and in vivo, and this effect may be through down-regulating the expression of NF-κB and MMP-9.