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目的观察用冷开水和灭菌水作为氧气湿化液使用过程中污染情况,规范氧气湿化液的使用。方法通过采集不同使用时间的湿化液样品进行细菌学检验,了解其污染情况。结果使用不足24 h的冷开水和无菌水检测到细菌生长阳性率分别为36.4%和37.9%,二者阳性率无统计学意义。使用24 h,冷开水细菌培养阳性率增加到44.4%,无菌水检测阳性率为30.3%。两种水中污染菌中,有60%是革兰阳性杆菌,均检出铜绿假单胞菌、凝固酶阴性葡萄球菌和不动杆菌。结论冷开水和无菌水作为氧气湿化液在使用期限和质量方面均无差别,可以实际使用。但在使用过程中应加强管理。
Objective To observe the use of cold water and sterile water as oxygen wetting fluid during the use of pollution, regulate the use of oxygen wetting fluid. Methods Bacteria tests were carried out on samples of wetting fluid collected at different usage times to understand the contamination. Results The positive rate of bacterial growth was 36.4% and 37.9% in cold water and sterile water, respectively, which was less than 24 hours. The positive rate of the two bacteria was not statistically significant. With 24 h, the positive rate of cold water bacterial culture increased to 44.4%, the positive rate of sterile water was 30.3%. Of the two waterborne contaminants, 60% were gram-positive bacilli, and P. aeruginosa, coagulase-negative staphylococci and Acinetobacter were detected. Conclusion Cold water and sterile water as oxygen wetting fluid in terms of life and quality are no different, can be used actually. However, the use of the process should be strengthened management.