目的:分析一株从长爪沙鼠盲肠分离的野生鼠疫噬菌体的生物学特性。方法:噬菌体来源于内蒙古长爪沙鼠鼠疫自然疫源地一只自毙长爪沙鼠，自盲肠分离。透射电镜观察噬菌体形态，分析噬菌体最佳感染复数，一步生长曲线，对温度和酸碱度的稳定性，对氯仿、乙醇、异丙醇和紫外线的敏感性，宿主谱，以及对鼠疫菌野毒株的裂解性。结果:噬菌体单噬菌斑直径约为10.00 mm，电镜观察噬菌体头部呈二十面体立体对称，由一直径约为51.72 nm的头部和长为10.00 nm的尾部组成。噬菌体最佳感染复数为0.01，潜伏期约为20 min，爆发期为20~100 min，爆发量为200 PFU/cell。噬菌体对50 ℃以下耐受良好，在pH 5~12的条件下能保持较高活性。噬菌体对氯仿、10%乙醇和异丙醇耐受良好，紫外线照射2 min即可杀灭大部分噬菌体。噬菌体宿主谱窄，专一性强，亦能裂解鼠疫菌野毒株。结论:该株鼠疫噬菌体分类归短尾噬菌体，有辅助诊断鼠疫菌的应用潜力，丰富了野生鼠疫噬菌体数据库，为野生鼠疫噬菌体的研究和应用奠定基础。“,”Objective:A strain of n Yersinia pestis phage was isolated from the cecum of n Meriones unguiculatus, and its biological characteristics were studied.n Methods:The phage was isolated from the cecum of a n Meriones unguiculatus which died in the n Meriones unguiculatus natural foci of plague in Inner Mongolia. Phage morphology was observed by transmission electron microscopy (TEM). The optimal multiplicity of infection (MOI), one-step growth curve, stability of the phage to temperature and acidity and alkalinity, sensitivity to chloroform, ethanol, isopropanol and ultraviolet rays, host range of phage lysis and lysis of the phage to wild strains of n Yersinia pestis were analyzed.n Results:The diameter of the phage plaque was about 10.00 mm. The head of the phage showed icosahedral symmetry under TEM, and the head was composed of a head with a diameter of 51.72 nm and a tail with a length of 10.00 nm. The MOI was 0.01, the incubation period was about 20 min, the outbreak period was 20-100 min and the outbreak amount was 200 PFU/cell. Under the condition of pH 5-12, it could maintain high activity, not sensitive to chloroform, 10% ethanol and isopropanol. Ultraviolet irradiation for 2 min could kill most of the phages. The host spectrum of the phage was narrow, and with strong specificity. It could lyse wild strains of n Yersinia pestis exclusively.n Conclusions:The phage is classified as family n Podoviridae, which has a potential to assist the diagnosis of n Yersinia pestis, enriches the database of n Yersinia pestis phages and lays a foundation for the research and application of n Yersinia pestis phages.n