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供试土壤采自桃源站红壤旱地稻草覆盖定位试验,利用末端限制性片段多态性(T-RFLP)分子技术研究不同稻草覆盖量对土壤中含phzF基因的荧光假单胞菌种群组成和多样性的影响。结果表明,与化肥处理相比,低量稻草覆盖(LRS)对phzF基因组成的影响较小,而中量和高量稻草覆盖(MRS和HRS)显著改变了phzF基因T-RFs的相对丰度,使含phzF基因的优势荧光假单胞菌种群结构发生明显变化。RDA分析表明,在稻草覆盖条件下土壤速效钾含量是影响phzF基因组成结构的主导因子。每公顷覆盖10000 kg稻草是有利于土壤荧光假单胞菌形成良好结构的稻草用量。研究结果为进一步揭示稻草覆盖对土壤抗病原微生物的影响机理提供了重要的依据。
The test soil was collected from the straw cover locating experiment of red soil dryland in Taoyuan Station. The effects of different straw mulching amount on the population of Pseudomonas fluorescens containing phzF gene in soil were studied by T-RFLP molecular technique And diversity effects. The results showed that LRS had little effect on phzF gene composition compared with chemical fertilization, while moderate and high amounts of straw coverage (MRS and HRS) significantly changed the relative abundance of phzF gene T-RFs , The dominant structure of phzF gene containing Pseudomonas fluorescens population structure changes significantly. RDA analysis showed that soil available K content under straw coverage was the dominant factor affecting the phzF gene composition. Covering 10,000 kg of straw per hectare is the amount of straw that is conducive to the formation of a well-structured Pseudomonas aeruginosa. The results provide an important basis for further revealing the mechanism of rice straw coverage on soil anti-pathogenic microorganisms.