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目的:探讨四跨膜蛋白CD151在肝内胆管细胞癌中的表达及与临床病理特征的关系。方法:应用RT-PCR与Westernblot检测36例肝内胆管细胞癌标本中四跨膜蛋白CD151的表达,免疫组织化学检测100例胆管癌组织中四跨膜蛋白CD151的表达,分析其与病理特征的关系。结果:18例早期复发肝内胆管细胞癌组织中CD151mRNA的表达明显高于未复发组(P<0.05);免疫组织化学检测显示CD151蛋白阳性表达率为61%(61/100),其表达程度与肿瘤大小、组织分化程度、TNM分期及淋巴结转移有关;肿瘤越大、分化程度越差、TNM分期高及有淋巴结转移者CD151表达越高(P<0.05)。结论:肝内胆管细胞癌CD151表达与其恶性表型相关,可能参与其侵袭和转移。
Objective: To investigate the expression of four-transmembrane protein CD151 in intrahepatic cholangiocarcinoma and its relationship with clinicopathological features. Methods: The expression of four-transmembrane protein CD151 in 36 specimens of intrahepatic cholangiocarcinoma was detected by RT-PCR and Western blot. The expression of four-transmembrane protein CD151 in 100 specimens of cholangiocarcinoma was detected by immunohistochemistry. relationship. Results: The expression of CD151 mRNA in 18 cases of early recurrence intrahepatic cholangiocarcinoma was significantly higher than that in non-recurrence group (P <0.05). Immunohistochemistry showed that the positive rate of CD151 protein was 61% (61/100) It was related to tumor size, histological grade, TNM stage and lymph node metastasis. The larger the tumor size was, the worse the differentiation degree was. The higher the TNM stage and lymph node metastasis were (P <0.05). Conclusion: The expression of CD151 in intrahepatic cholangiocarcinoma correlates with its malignant phenotype, which may be involved in its invasion and metastasis.