论文部分内容阅读
从红豆杉（Ｔａｘｕｓｃｈｉｎｅｎｓｉｓ（Ｐｉｌｇ．）Ｒｅｈｄ．）的嫩茎及针叶诱导出愈伤组织，对愈伤组织培养及细胞悬浮培养进行了研究。利用ＨＰＬＣ方法测定了它们合成紫杉醇的能力。发现了能够提高培养细胞生长速率及紫杉醇含量的一些因子。红豆杉愈伤组织及悬浮培养细胞的生长速率已分别达到０．２５ｇ／Ｌ．ｄ和０．２８ｇ／Ｌ．ｄ。而他们的紫杉醇含量分别是０．００２６％和０．０１２％。
Callus was induced from tender stems and needles of Taxus chinensis (Pilg.) Rehd. The callus culture and cell suspension culture were studied. Their ability to synthesize paclitaxel was determined by HPLC method. A number of factors have been found that increase the rate of cell growth and paclitaxel content. The growth rate of callus and suspension cultured cells reached 0.25g / L respectively. d and 0.28 g / L. d. Their paclitaxel content was 0.0026% and 0.012%, respectively.