增强UV-B辐射与干旱复合处理对小麦幼苗生理特性的影响

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为研究由于平流层臭氧层减薄紫外线 B辐射增强在干旱地区对春小麦生理特性的影响的特殊性 ,模拟平流层臭氧减少 2 0 %时辐射到地表的紫外线 B(UV-B,2 80~ 3 1 5 nm)的增强和水分胁迫 (-0 .5 Mpa,聚乙二醇 PEG-60 0 0处理获得 ) ,通过测定两种胁迫下春小麦 (Triticum aestivum L.)叶绿素含量、类黄酮含量、水势、细胞膜相对透性、超氧歧化酶(SOD)活性及丙二醛 (MDA)含量 ,研究了增强 UV-B辐射和水分胁迫复合作用对温室种植的小麦幼苗生理生化的影响。实验结果表明 ,虽然水分胁迫和 UV-B辐射单独或复合处理都使春小麦的叶绿素含量降低 ,但仅 UV-B辐射增强单独处理显著地降低小麦叶绿素 a、b和总叶绿素的含量 ,而水分胁迫以及复合处理对叶绿素的含量的降低作用不显著。两种胁迫无论是单独作用还是复合作用均能使类黄酮含量升高 ,并且处理第 3天比第 1天高出近 5 0 % ,复合处理下类黄酮的含量大于两个因子单独处理。UV-B辐射和水分胁迫处理 1 d对春小麦叶片的相对电导率的影响不明显 ,处理 3 d后两种胁迫下相对电导率均上升 ,表明膜透性增加 ,其中水分胁迫作用下增加尤其明显。膜质过氧化产物丙二醛的含量 ,在两种因子单独和复合作用下都升高 ,说明膜的生理功能受到了一定的不利影响。活性氧清除剂超氧化物 In order to study the peculiarity of the effect of enhanced ultraviolet B radiation in the stratosphere on the physiological characteristics of spring wheat in the arid region, we simulated the UVB (UV-B, 280 ~ 31 5 nm) and water stress (-0.5 Mpa, polyethylene glycol PEG-60 0 0 treatment). By measuring the chlorophyll content, flavonoid content, water potential, The relative permeability of cell membrane, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were studied. The effects of enhanced UV-B radiation and water stress on physiological and biochemical characteristics of wheat seedlings planted in greenhouse were studied. The results showed that only chlorophyll a, b and total chlorophyll content in wheat significantly decreased with UV-B radiation alone, although the chlorophyll content of spring wheat decreased with water stress and UV-B radiation alone or in combination. However, the water stress And the effect of compound treatment on chlorophyll content was not significant. The two kinds of stress could increase the content of flavonoids either alone or in combination, and were nearly 50% higher than the first day on the third day of treatment. The content of flavonoids in the composite treatment was greater than the two factors alone. The effect of UV-B radiation and water stress for 1 d on the relative conductivity of spring wheat leaves was not obvious. After 3 days of treatment, the relative electrical conductivity of the two kinds of stresses increased, indicating that the membrane permeability increased, especially under water stress. . Membrane peroxidation of malondialdehyde content, in the two factors alone and under the combined effects are increased, indicating that the physiological function of the membrane has been some adverse effects. Active oxygen scavenger superoxide
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