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目的分析2010年信阳市手足口病实验室检测结果,明确2010年信阳市手足口病原学特征,为制定预防控制措施提供依据。方法采集2010年信阳市各县区(市)送检的手足口患者的425份各类样本,应用RT-PCR技术和荧光PCR技术检测样本中的肠道病毒71型(EV71),柯萨奇病毒A组(CA16)。结果 425份各类样本中,检测到EV71特异性片段141份,CA16特异性片段46份,EV71和CA16核酸同时阳性12份,其它肠道病毒阳性133份,疱疹液、粪便、肛拭阳性检出率较高,均在60.00%以上;应用RT-PCR技术检出样本阳性率为67.80%,应用荧光PCR技术检出样本阳性率为83.85%,荧光PCR方法检出率明显高于RT-PCR(χ2=11.71,P<0.001)。结论 EV71是2010年信阳市手足口病的优势病原体;应用荧光PCR技术检测样本能提高检出率;疱疹液、粪便、肛拭是实验室诊断较敏感的标本。
Objective To analyze the laboratory test results of hand-foot-mouth disease in Xinyang City in 2010 and to clarify the etiological characteristics of hand-foot-mouth disease in Xinyang City in 2010 so as to provide basis for the prevention and control measures. Methods A total of 425 samples of hand, foot and mouth were collected from all counties and districts of Xinyang City in 2010. The samples of enterovirus 71 (EV71), coxsackie Virus A group (CA16). Results Among 425 samples of various types, 141 EV71-specific fragments, 46 CA16-specific fragments, 12 EV71 and CA16 nucleic acids were positive at the same time, 133 were positive by other enteroviruses, herpes simplex, The positive rate was 67.80% by RT-PCR, the positive rate was 83.85% by fluorescence PCR, and the detection rate by fluorescence PCR was significantly higher than that by RT-PCR (χ2 = 11.71, P <0.001). Conclusion EV71 is the predominant pathogen of hand-foot-mouth disease in Xinyang City in 2010. Fluorescent PCR can increase the detection rate. Herpes fluid, faecal and anal swabs are more sensitive laboratory specimens.