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Objectives: To investigate the cause and preventive measures of skin infection of the newborn of direct rooming-in, in order to provide practical basis and reduce the incidence of skin infection. Method: select 2100 direct rooming-in newborns of obstetric department in our hospital from March 2012 to February 2013, using random sampling method to choose one of the 1050 cases as the intervention group, and the remaining 1050 cases as routine nursing group. After the birth, routine nursing group used 75% alcohol to disinfect the umbilical around, and ligation, dressing and other routine nursing care. Early intervention group took early nursing intervention to prevent skin infections on the basis of routine nursing group. Results: routine nursing group diagnosed 22 cases of neonatal skin infections, and the incidence rate was 2.10%. 7 cases in early nursing group were diagnosed as neonatal skin infections, and the incidence rate was 0.67%. Neonatal skin infections of two groups had significant difference (X2=12.73, P0.05). Conclusion: it can effectively reduce the occurrence of neonatal infections of the skin, and improve the quality of nursing care by understanding the causes of neonatal skin infection of direct rooming-in, and the composition of pathogenic bacteria, and taking active preventive measures.