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试验结果表明,带子叶接种显著影响F_1代黄瓜津春四号外植体的生长和开花;培养基、外源生长物质和外植体种类对黄瓜试管苗生长和开花同等重要,但各因素不同水平间差异显著;试验分析结果表明,以生长点接种MS+1.0毫克/升ZT+0.5毫克/升NAA培养基进行试管开花试验是最理想的;1.0毫克/升KT或0.5毫克/升BA+0.5毫克/升NAA对黄瓜外植体的愈伤化无协同作用;切割后子叶(3×3平方毫米)外植体培养不形成花芽;黄瓜子叶叶腋与真叶叶腋存在着同样分化叶芽和花芽的能力。
The results showed that inoculation with cotyledons significantly affected the growth and flowering of explants of F_1 generation cucumber Jinchun IV. The culture media, exogenous growth substances and explants types were equally important for the growth and flowering of cucumber plantlets, but different levels The results showed that the optimal flowering test was inoculated with MS + 1.0 mg / L ZT + 0.5 mg / L NAA medium at growth point; 1.0 mg / L KT or 0.5 mg / L BA + 0.5 There was no synergistic effect of mg / L NAA on callus induction of cucumber explants; cotyledons (3 × 3 mm 2) explants did not form flower buds after cutting; the same differentiated leaf buds and flower buds existed in axillary axils of cucumber cotyledons ability.