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[目的]了解福建省肠炎沙门菌的流行状况,更好地开展监测和防控工作。[方法]2012年对2009～2010年分离自福建省沙门菌病患者的30株肠炎沙门菌菌株采用分子分型及检测毒力基因的方法进行分析。[结果]30株肠炎沙门菌中,按照100%的相似度酶切图谱分为13个PFGE型,其中P1型5株,P4型7株,为优势型别。根据TENOVER原则,可得到1个优势基因群G1(包含P1～P7型),合计23株,占76.67%。prgH、sopB、invA、sitC、sifA、iroN基因均阳性,spvB基因阳性的13株(占43.33%),pefA基因阳性的22株(占73.33%)。[结论]2009～2010年福建省肠炎沙门菌PFGE分型呈现多态性,存在1个优势基因群G1,携带多种毒力基因。
[Objective] To understand the prevalence of Salmonella enteritidis in Fujian Province and to carry out better monitoring and prevention and control. [Method] Thirty strains of Salmonella enteritidis isolated from Fujian Province from 2009 to 2010 were analyzed by molecular typing and virulence detection. [Result] Thirty Salmonella enteritidis strains were divided into 13 PFGE types according to the 100% similarity digest pattern, in which 5 strains were P1 type and 7 strains were P4 type, which was the predominant type. According to the principle of TENOVER, one dominant gene group G1 (including P1 ~ P7 type) can be obtained, accounting for a total of 23 strains (76.67%). 13 strains (accounting for 43.33%) of spvB gene positive, 22 strains (accounting for 73.33%) of pefA gene positive were positive for prgH, sopB, invA, sitC, sifA and iroN. [Conclusion] The PFGE genotypes of Salmonella enteritidis in Fujian Province from 2009 to 2010 showed polymorphism, and one dominant gene group G1 existed, carrying multiple virulence genes.