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为了对林下山参的不同部位进行鉴别,本研究采用基于UHPLC-QTOF/MS的植物代谢组学技术,并结合PCA和OPLS-DA分析方法对林下山参叶、主根、侧根、芦头等4个部位的代谢物进行研究。PCA得分图显示林下山参叶、主根、侧根和芦头被明显分为4类,表明林下山参不同部位之间化学成分差异显著。从林下山参4个不同部位中共检测到81个主要代谢物,其中包括林下山参叶与根茎(主根、侧根、芦头)之间的差异标志物9个,林下山参主根与侧根、主根与芦头之间的差异标志物各7个,通过将这些代谢物的保留时间、精确分子量及碎片离子与对照品和相关人参属文献报道的化合物进行匹配,最终鉴定或初步推断出70个代谢物。本研究建立了一种快速、准确、可靠的林下山参不同部位的鉴别方法,同时也为其他中药材质量评价提供了一种新的思路。
In order to identify different parts of Panax ginseng, we used plant metabonomics based on UHPLC-QTOF / MS, and combined with PCA and OPLS-DA analysis methods, Site of metabolites to study. The PCA score showed that under the tree ginseng, the main roots, lateral roots and reed heads were apparently divided into four groups, indicating that there were significant differences in the chemical compositions among the different parts of the understory ginseng. Eighty-one main metabolites were detected from 4 different parts of Grifola frondosa, including 9 differential markers of leaf and rhizome (main root, lateral root and reed head), the main root and lateral root of Grifola frondosa, And Rehmannia, each of seven markers were identified or initially inferred by matching the retention times, exact molecular weights and fragment ions of these metabolites with the control and related Panax species compounds in the literature Things. This study established a rapid, accurate and reliable identification method of different parts of Grifola frondosa, and also provided a new idea for the quality evaluation of other Chinese medicinal materials.