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目的微束等离子喷涂由于喷涂功率低,喷涂温度低,可以解决等离子喷涂羟基磷灰石分解这一问题。因此,本研究以大气等离子喷涂羟基磷灰石涂层(APS-HA)为对照组,微束等离子喷涂羟基磷灰石涂层(MPS-HA)为实验组,研究微束等离子喷涂羟基磷灰石涂层(MPS-HA)的细胞生物相容性。方法以MC3T3-E1小鼠成骨细胞为实验细胞,将成骨细胞接种到两种喷涂方法的生物材料表面,采用扫描电镜(SEM)、四唑盐比色法(MTT)、碱性磷酸酶检测(ALP),研究成骨细胞在两种生物材料表面形态、粘附、增殖、分化的影响。结果 APS-HA组和MPS-HA组均具有良好的生物相容性,MPS-HA组更有利于成骨细胞的生长、粘附、增殖,能促进成骨细胞向成熟的表型分化。结论微束等离子喷涂羟基磷灰石涂层(MPS-HA)与成骨细胞具有良好的相容性,材料表面有利于成骨细胞的粘附和增殖。为其成为新型口腔种植体提供理论依据。
Purpose Micro-beam plasma spraying Due to the low spraying power and low spraying temperature, the problem of plasma sprayed hydroxyapatite decomposition can be solved. Therefore, in this study, plasma-sprayed hydroxyapatite coating (APS-HA) was used as a control group, and micro-plasma sprayed hydroxyapatite coating (MPS-HA) Cell Biocompatibility of Stone Coating (MPS-HA). Methods Using osteoblasts from MC3T3-E1 mice as experimental cells, osteoblasts were inoculated onto the surface of biomaterials by two spraying methods. Scanning electron microscopy (SEM), tetrazolium salt colorimetry (MTT) and alkaline phosphatase (ALP) to study the effects of osteoblasts on the morphology, adhesion, proliferation and differentiation of two biomaterials. Results Both APS-HA group and MPS-HA group had good biocompatibility. MPS-HA group was more conducive to the growth, adhesion and proliferation of osteoblasts, and promoted the osteoblastic phenotype differentiation. Conclusion Micro-plasma sprayed hydroxyapatite coating (MPS-HA) has good compatibility with osteoblasts. The surface of the material is conducive to the adhesion and proliferation of osteoblasts. Provide a theoretical basis for becoming a new type of dental implants.