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聚羟基脂肪酸酯(Polyhydroxyalkanoates,PHAs)作为一类新型的生物高分子材料,因其多样的材料性质与高度的生物可降解性日益受到关注。使用乳酸链球菌素(Nisin),一种被公认为安全的天然食品防腐剂,制备了具有高效、持久抗菌效应的PHA塑料。首先采用溶剂浇铸的方法将Nisin整合到正3-羟基丁酸-3羟基己酸共聚酯(PHBHHx),一种具备高度生物相容性的PHA中,从而获得了具有抗菌效应的PHBHHx薄膜。激光共聚焦显微镜观察表明Nisin在PHBHHx中呈颗粒状均匀分布。随后以条件致病菌藤黄微球菌Micrococcus luteus为测试菌株,通过琼脂扩散法,测定PHBHHx薄膜抗菌效应对Nisin含量的依赖关系;在液体培养条件下测量PHBHHx薄膜的Nisin释放效果与抗菌效应。结果表明Nisin可从PHBHHx薄膜顺利释放且Nisin的含量高于25μg/g时即表现出显著的抑菌效果且可长时间维持。该研究为工业化生产具有抗菌效应的PHA奠定了重要的技术基础,拓展了PHA在医学和食品领域的应用潜力。
Polyhydroxyalkanoates (PHAs), as a new type of biopolymer, are attracting more and more attention because of their diverse material properties and high biodegradability. Using Nisin, a natural food preservative known to be safe, PHA plastics with an effective, long-lasting antimicrobial effect have been prepared. First, Nisin was incorporated into PHBHHx, a highly biocompatible PHA by solvent casting, to obtain an antimicrobial PHBHHx film. Laser confocal microscopy showed that Nisin was uniformly distributed in PHBHHx. Subsequently, the conditional bacterium Micrococcus luteus was used as the tested strain, and the dependence of Nisin content on the antibacterial effect of PHBHHx film was determined by agar diffusion method. The Nisin release effect and antibacterial effect of PHBHHx film were measured under liquid culture conditions. The results show that Nisin can be smoothly released from the PHBHHx film and exhibits significant antibacterial effect when the Nisin content is higher than 25 μg / g and can be maintained for a long time. The research laid an important technical foundation for the industrial production of PHA with antibacterial effect and expanded the potential of PHA in the field of medicine and food.