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目的:观察落花生枝叶干预后对阿尔茨海默病转基因小鼠的影响,探讨花生枝叶在氧化损伤中潜在的脑保护作用。方法:实验分三组,正常组、对照组和落花生干预组。实验用落花生枝叶水煎液对2月龄的阿尔茨海默病转基因小鼠每天灌胃连续6月,期间每三个月给予水迷宫行为学测试各一次,并在给药结束后处死小鼠,进行海马组织形态学和活性氧检测。结果:二次水迷宫检测中正常组、对照组和落花生枝叶组间无统计学差异(P>0.05);在海马组织形态学检测中,与对照组相比,落花生枝叶组海马区细胞相对胞质开始丰满,且细胞数目也有所增加,细胞带排列逐渐有序;而在凋亡细胞检测中,与对照组相比,落花生枝叶组凋亡细胞数量明显降低,具有统计学差异(P<0.05);在组织ROS测定中,落花生枝叶组与对照组比较有显著性差异(P<0.05)。结论:落花生枝叶对对阿尔茨海默病转基因小鼠氧化损伤具有潜在保护作用。
Objective: To observe the effects of branches and leaves of peanut on the Alzheimer’s disease transgenic mice and to explore the potential brain protective effect of peanut branches and leaves on oxidative damage. Methods: The experiment was divided into three groups, normal group, control group and groundnut intervention group. Experimental peanut branches and leaves decoction of 2-month-old Alzheimer’s disease transgenic mice were fed daily for 6 months, every three months during the water maze to conduct behavioral tests once, and after the end of the mice were sacrificed , Hippocampal histomorphology and reactive oxygen species detection. Results: There was no significant difference in the secondary water maze test between the normal group, the control group and the peanut branch and leaf group (P> 0.05). In hippocampal histopathological examination, compared with the control group, The quantity of apoptotic cells in the branches and leaves of peanut decreased significantly compared with the control group (P <0.05), and the numbers of apoptotic cells in the branches and leaves of peanut were significantly decreased ). In the tissue ROS assay, there were significant differences between the peanut branch and the control group (P <0.05). Conclusion: The peanut branches and leaves have the potential protective effect on the oxidative damage of Alzheimer’s disease transgenic mice.