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目的从T细胞亚群及细胞因子的角度探究当归芍药散对慢性盆腔炎大鼠模型的作用机制。方法采用混合菌感染合并机械损伤法制备慢性盆腔炎大鼠模型。60只雌性大鼠随机分为正常组,模型组,金刚藤胶囊组,当归芍药散高、中、低剂量组。用药14 d后,观察子宫病理组织改变;流式细胞术测定外周血CD3、CD4、CD8细胞,辅助性淋巴细胞(Th细胞),细胞毒性淋巴细胞(Tc细胞)数量百分比;测定血清中一氧化氮(NO)、白细胞介素(IL)-4、IL-10的含量。结果光学显微镜观察显示,当归芍药散高、中剂量组可以降低大鼠子宫内膜的炎症细胞浸润,促进病变上皮细胞增生修复;当归芍药散高、中剂量组可以显著升高Th细胞、CD3细胞、CD4细胞以及Th/Tc、CD4/CD8比值(P<0.01,P<0.05),降低Tc细胞、CD8细胞(P<0.01,P<0.05);当归芍药散高、中剂量组可以显著升高IL-4、IL-10含量,降低NO含量(P<0.05);与金刚藤胶囊组比较,高剂量组显著升高IL-4含量(P<0.01),降低NO含量(P<0.05)。结论当归芍药散可能通过改善免疫状态,降低炎症反应以发挥治疗慢性盆腔炎的作用。
Objective To explore the mechanism of action of Danggui Shaoyao San on chronic pelvic inflammatory disease rat model from the perspective of T cell subsets and cytokines. Methods Chronic pelvic inflammatory disease rats were prepared by mixed bacterial infection combined with mechanical injury. 60 female rats were randomly divided into normal group, model group, Jingangtu capsule group, Danggui Shaoyao San high, medium and low dose groups. The pathological changes of the uterus were observed 14 d after treatment. The percentage of CD3, CD4, CD8, T lymphocytes and Tc cells in peripheral blood were measured by flow cytometry. Nitrogen (NO), interleukin (IL) -4, IL-10 content. Results The optical microscope observation showed that the Danggui Shaoyao San high and medium dose groups could reduce the infiltration of inflammatory cells in the rat endometrium and promote the proliferation and repair of the diseased epithelial cells. While the Danggui Shaoyao San high and medium dose groups could significantly increase Th cells and CD3 cells (P <0.01, P <0.05), Tc and CD4 / CD8 ratio (P <0.01, P <0.05), and decreased Tc and CD8 IL-4, IL-10 content and NO content decreased (P <0.05). Compared with Jinguetang capsule group, IL-4 content increased significantly (P <0.01) and NO content decreased (P <0.05) Conclusion Danggui Shaoyao San may play an active role in the treatment of chronic pelvic inflammatory disease by improving the immune status and reducing the inflammatory reaction.