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为了研究凝血酶受体在血管平滑肌细胞增埴中的作用 ,探讨反义凝血酶受体基因序列对大鼠血管平滑肌细胞增殖的影响 ,通过培养SD大鼠胸主动脉血管平滑肌细胞 ,以3 H TdR掺入率作为评价反义凝血酶受体基因序列抑制血管平滑肌细胞增殖的指标 ;用反转录聚合酶链反应检测反义凝血酶受体基因序列抑制血管平滑肌细胞凝血酶受体mRNA的表达 ;用Western blot检测反义凝血酶受体基因序列抑制血管平滑肌细胞凝血酶受体蛋白质的表达 ;用3 H 肌醇掺入率检测反义凝血酶受体序列抑制血管平滑肌细胞磷酸肌醇代谢的影响。结果发现 ,反义凝血酶受体基因序列明显抑制血管平滑肌细胞的增殖 (与对照组相比 ,P <0 .0 5 ) ;明显抑制血管平滑肌细胞的凝血酶受体mRNA和蛋白的表达 ;明显抑制了血管平滑肌细胞磷酸肌醇代谢。提示反义凝血酶受体基因序列明显抑制血管平滑肌细胞的增殖 ;凝血酶受体反义序列明显抑制血管平滑肌细胞的增殖是通过抑制凝血酶受体基因的表达 (特别是通过抑制DNA、mRNA和蛋白的表达 ) ,抑制细胞内信号传递来完成
In order to study the role of thrombin receptor in the proliferation of vascular smooth muscle cells and explore the effect of antisense thrombin receptor gene sequence on the proliferation of rat vascular smooth muscle cells, thoracic aorta vascular smooth muscle cells were cultured in vitro, TdR incorporation rate as an indicator to evaluate the antisense thrombin receptor gene sequence inhibition of vascular smooth muscle cell proliferation; reverse transcriptase polymerase chain reaction detection of antisense thrombin receptor gene sequence inhibition of vascular smooth muscle cell thrombin receptor mRNA expression ; Antisense thrombin receptor gene sequence was detected by Western blot to inhibit the expression of thrombin receptor protein in vascular smooth muscle cells; 3 H myo-inositol incorporation rate detection of antisense thrombin receptor sequence inhibition of vascular smooth muscle cell phosphoinositide metabolism influences. The results showed that antisense thrombin receptor gene sequence significantly inhibited the proliferation of vascular smooth muscle cells (compared with the control group, P <0 05); significantly inhibited the expression of thrombin receptor mRNA and protein in vascular smooth muscle cells; Inhibited the phosphoinositide metabolism of vascular smooth muscle cells. It is suggested that the antisense thrombin receptor gene sequence significantly inhibits the proliferation of vascular smooth muscle cells. Antisense prothrombin receptor inhibits the proliferation of vascular smooth muscle cells by inhibiting the expression of thrombin receptor genes (especially by inhibiting DNA, mRNA and Protein expression), inhibition of intracellular signaling to complete