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目的:观察癌干细胞CD44+/ALDH+在结直肠癌组织中的表达,并探讨其临床病理学意义.方法:利用组织芯片仪制作组织芯片,通过免疫组织化学染色,免疫组织化学双重染色观察结直肠癌组织和不同组织中CD44+,ALDH+,CD44+/ALDH+的表达.结果:CD44,ALAH1在结直肠癌中的表达明显高于正常肠黏膜和伴不典型增生的管状腺瘤(均P<0.05);在结直肠癌中,CD44和ALDH1的表达率与癌组织的病理分级、浸润、淋巴结转移有关(均P<0.05);CD44+/ALDH+在结直肠癌中的表达明显高于正常肠黏膜组织和伴不典型增生的管状腺瘤(均P<0.05),随着癌分化程度的降低,表达率增加.结论:CD44+/ALDH+可作为结直肠癌干细胞有价值的标志物,可作为结直肠癌早期和预防复发转移的可靠指标.
OBJECTIVE: To observe the expression of CD44 + / ALDH + in colorectal cancer tissues and to explore its clinicopathological significance.Methods: The tissue microarray was used to make tissue microarray to observe the expression of CD44 + / ALDH + in colorectal cancer by immunohistochemical staining and immunohistochemical double staining The expression of CD44, ALAH1 in colorectal cancer was significantly higher than that in normal intestinal mucosa and atypical hyperplastic tubular adenoma (all P <0.05). In the presence of CD44 +, ALDH +, CD44 + / ALDH + The expression of CD44 and ALDH1 in colorectal cancer was correlated with the pathological grade, infiltration and lymph node metastasis (all P <0.05). The expression of CD44 + / ALDH + in colorectal cancer was significantly higher than that in normal mucosa (P <0.05), and the expression rate of CD44 + / ALDH + increased as the degree of differentiation decreased.Conclusion: CD44 + / ALDH + can be used as a valuable marker of colorectal cancer stem cells and can be used as early stage and prevention of colorectal cancer Reliable indicators of recurrence and metastasis.