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目的 :检测骨髓细胞移植在急性心肌梗死大鼠模型中对细胞保护性蛋白HSP32和HSP70表达水平的影响 ,探讨细胞移植早期改善缺血心脏功能的可能机制。方法 :通过结扎冠状动脉左前降支制备大鼠心肌梗死模型 ,直视下心外膜注射 5× 10 6骨髓单个核细胞 ;利用免疫荧光和RT -PCR技术检测HSP32和HSP70在术后 1d、3d、7d和 14d的表达变化和移植细胞的分化情况 ;通过超声心动图分析心脏功能左室射血分数 (EF值 )和缩短分数(FS值 )。结果 :免疫荧光结果显示 ,缺血心肌中HSP32和HSP70的表达在移植组明显增强 ,并表达于部分移植细胞内。RT -PCR结果表明 ,移植组HSP32和HSP70的mRNA表达明显高于对照组 ,术后 3d达到高峰 ,比对照组分别增加 5 .0倍和 2 .9倍 (P <0 .0 1)。术后 7d ,移植组EF值和FS值明显高于同期对照组 (14 %和 2 2 % ,P <0 .0 5 ) ;术后 14d ,可在移植的骨髓细胞中检测到心肌细胞和血管内皮细胞特异性蛋白的表达 ,EF值和FS值进一步增加。结论 :骨髓细胞移植早期 ,细胞移植能够上调细胞保护性蛋白的表达 ,促进急性缺血心脏功能恢复
OBJECTIVE: To investigate the effect of bone marrow cell transplantation on the expression of protective proteins HSP32 and HSP70 in rat models of acute myocardial infarction (AMI) and to explore the possible mechanism of improving cardiac function in early stage of cell transplantation. Methods: Myocardial infarction model was established by ligation of the left anterior descending artery of the coronary artery. The epicardium was injected with 5 × 10 6 bone marrow mononuclear cells under direct vision. The expression of HSP32 and HSP70 were detected by immunofluorescence and RT-PCR at 1 day, 7d and 14d, and the differentiation of transplanted cells. The left ventricular ejection fraction (EF) and shortening fraction (FS) of cardiac function were analyzed by echocardiography. Results: Immunofluorescence results showed that the expression of HSP32 and HSP70 in ischemic myocardium was significantly increased in the transplantation group and was expressed in some of the transplanted cells. The results of RT-PCR showed that the mRNA expression of HSP32 and HSP70 in the transplantation group was significantly higher than that in the control group, reaching the peak on 3d after the operation, increasing by 5.0 times and 2.9 times (P <0.01) respectively. At 7 days after operation, the EF and FS values in the transplantation group were significantly higher than those in the control group (14% and 22%, P <0.05). On the 14th day after transplantation, myocardial cells and blood vessels were detected in the transplanted bone marrow cells Endothelial cell-specific protein expression, EF and FS values were further increased. Conclusion: In the early stage of bone marrow cell transplantation, cell transplantation can up-regulate the expression of cytoprotective protein and promote the recovery of acute ischemic heart function