利用RAPD对抗卷叶病品系CNH123、CNH1012和感病品系CNH1020、CNH120分别建立了抗、感病多态带,用80个引物进行PCR扩增。引物OPC02在抗病品系CNH123和CNH1012中得到1700bp的特征片段,建立了10个抗病及感病DNA库并进行混合分组分析,在F2群体用OPC02重现了上述特征片段,并将该片段改造为SCAR标记,设计引物对为5’ GTGAG GCGTCAGAGGGAT 3′(正向)and5′ GTTGC CGTGCACTAGGCT 3′(反向)。利用Mapmaker软件得到10个F2分离群体的RAPD分离图谱。 The resistant and susceptible polymorphic bands of RAPD lines CNH123, CNH1012 and CNH1020 and CNH120 were established respectively by RAPD and 80 primers were used for PCR amplification. Primer OPC02 obtained 1700bp characteristic fragment from resistant strain CNH123 and CNH1012, established 10 resistance and susceptible DNA pools and analyzed by mixed grouping. The F2 fragment was reconstructed by OPC02 and the fragment was transformed For SCAR labeling, the primer pairs were designed to be 5 ’GTGAG GCGTCAGAGGGAT 3’ (forward) and 5 ’GTTGC CGTGCACTAGGCT 3’ (reverse). Mapmaker software was used to obtain RAPD profiles of 10 F2 segregating populations.