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目的：观察４月胎肝提取物对ＱＧＹ－７７０３、ＢＥＬ－７４０２、ＳＭＭＣ－７７２１人肝癌细胞和ＨｅｐＡ小鼠肝瘤细胞增殖和分化的影响。方法：从４月胎肝中制备提取物，采用［３Ｈ］－ＴｄＲ掺入法和ＭＴＴ比色法。结果：１００～５００ｍｇ／Ｌ的胎肝提取物能明显抑制ＱＧＹ－７７０３、ＢＥＬ－７４０２、ＳＭＭＣ－７７２１、ＨｅｐＡ等肝癌细胞的ＤＮＡ合成和脱氢酶活力。对ＤＮＡ合成的半数抑制浓度（ＩＣ５０）分别为３４６．９３、１５１．１８、１８２．３７、２３１．０３ｍｇ／Ｌ；对脱氢酶活力的抑制弱于对ＤＮＡ合成的抑制，ＩＣ５０分别为１３１８．１、１５９９．２、７９３．８、８１３．１ｍｇ／Ｌ。结论：胎肝提取物能够抑制上述４种细胞增殖和促进分化。
OBJECTIVE: To observe the effect of April fetal liver extract on the proliferation and differentiation of QGY-7703, BEL-7402, SMMC-7721 hepatoma cells and HepA mouse hepatoma cells. METHODS: Extracts were prepared from fetal liver in April using [3H] -TdR incorporation and MTT colorimetry. Results: 100 ~ 500mg / L fetal liver extract could significantly inhibit DNA synthesis and dehydrogenase activity of QGY-7703, BEL-7402, SMMC-7721 and HepA cells. The half inhibitory concentration (IC50) of DNA synthesis was 346.93,151.18,182.37,231.03mg / L, respectively. The inhibition of dehydrogenase activity was weaker than the inhibition of DNA synthesis with IC50 of 1318, respectively. 1,1599.2,793.8,813.1 mg / L. Conclusion: Fetal liver extract can inhibit the above four kinds of cell proliferation and promote differentiation.