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[目的]研究溶藻特性及菌藻关系,为进一步研究溶藻细菌对水华的治理作用提供帮助。[方法]从富营养化水体中分离得到一株有高效溶藻效果的菌株(S7),研究了其对水华鱼腥藻(Anabaena flosaquae)的抑制效果、作用方式和不同环境因子对溶藻效果的影响,以及菌藻关系,并对菌株进行了菌体Poly-p的染色、革兰氏染色和分子鉴定。[结果]菌株投加量为藻液量的30%时,7d叶绿素a的去除率达到90%以上。pH为9,温度35℃下藻的去除率最高。S7菌株与水华鱼腥藻形成竞争共栖的生态关系,并通过分泌溶藻物质间接抑制水华鱼腥藻生长,且该物质具有一定的热稳定性。根据生理生化及16SrDNA序列分析鉴定,S7属于金黄杆菌属(Chryseobacterium)。[结论]该菌对水华鱼腥藻有较强的溶藻效果,且为聚磷菌。
[Objective] The research aimed to study the characteristics of algae and relationship between bacteria and algae and provide help for the further study on algae-algae treatment by algae bacteria. [Method] A strain (S7) with high efficiency of algicidal activity was isolated from eutrophic water, and its inhibitory effect on Anabaena flosaquae and the effect of different environmental factors on algicidal activity Effect and the relationship between bacteria and algae, and strains of bacteria Poly-p staining, Gram staining and molecular identification. [Result] The rate of chlorophyll a removal reached above 90% when the strain was added at 30% of the amount of algae liquid. The pH was 9 and the removal of algae was the highest at 35 ℃. S7 strain and Anabaena spawn competing for ecological relationship, and indirectly inhibit the growth of Anabaena sp. By secreting algae substance, and the substance has certain thermal stability. According to physiological and biochemical and 16S rDNA sequence analysis identified, S7 belongs to the genus Chryseobacterium (Chryseobacterium). [Conclusion] The bacterium had strong algicidal effect on Anabaena flos-aquae and was polyphosphate-accumulating bacteria.