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目的 研究小鼠肾脏发育过程中的细胞凋亡规律及形态学特点。 方法 应用光镜、电镜技术和TUNEL法分别对不同胚龄、生后日龄小鼠肾脏细胞凋亡进行了观察。 结果 皮质凋亡细胞多出现在生肾区S小体之间和肾小体内 ,凋亡高峰期在胚龄 14～ 18d之间。髓质凋亡细胞出现在肾小管上皮内 ,凋亡高峰期在生后 7d左右。超微结构观察可见皮质和髓质凋亡细胞主要表现为核固缩 ,染色质凝集 ,细胞皱缩。皮质和髓质凋亡细胞结局为 ,被邻近细胞吞噬 ,或脱落到肾小管腔内。 结论 小鼠肾脏发育过程中确有细胞凋亡 ;皮质中细胞凋亡与生肾区的出现和肾小体发育完善有关 ,髓质中细胞凋亡与髓质中肾小管和集合小管的发育完善有关
Objective To study the apoptosis and morphological characteristics of mouse kidney during development. Methods Apoptosis of kidney in different embryonic and postnatal mice was observed by light microscopy, electron microscopy and TUNEL. Results Cortical apoptotic cells appeared mostly in the S body between the nephrotic regions and in the renal bodies. The peak of apoptosis was between 14 and 18 days of embryo age. Medulla apoptotic cells in renal tubular epithelial cells, the peak of apoptosis in postnatal 7d or so. Ultrastructural observation shows that the cortical and medulla apoptotic cells mainly manifested as nuclear pyknosis, chromatin condensation, cell shrinkage. Cortical and medullary apoptotic cells end up engulfed by nearby cells or shed into the renal tubule. Conclusions Apoptosis is indeed observed during the development of kidney in mice. The apoptosis in cortex is related to the appearance of nephrotic region and the perfection of nephrotic development. The apoptosis of medulla and the development of tubular and collecting tubules in medulla related