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目的:研究流行性乙型脑炎(乙脑)患儿脑脊液、血清白介素1β(IL-1β)与神经元特异性烯醇酶(NSE)的变化及其临床意义。方法:50例乙脑患儿为观察组,按Glasgow评分分正常组17例,轻度组20例,重度组13例。检测极期、恢复期脑脊液及血清IL-1β、NSE含量,并与20例非中枢神经系统疾病患儿进行对照。IL-1β采用双抗体夹心酶联免疫法检测,NSE采用电化学发光法测定。结果:乙脑患儿脑脊液、血清中IL-1β及NSE含量在极期、恢复期皆比对照组显著增高(P均<0.01),且增高程度与病情严重程度相平行(P<0.01)。脑脊液与血清IL-1β含量及脑脊液与血清NSE含量分别呈正相关(P<0.01),脑脊液IL-1β与NSE含量及血清IL-1β与NSE含量亦分别呈正相关(P<0.01)。结论:脑脊液及血清IL-1β,NSE含量测定对乙脑患儿脑损伤程度及临床病情的判断有重要参考价值。
Objective: To study the changes and clinical significance of cerebrospinal fluid (CSF), interleukin-1β (IL-1β) and neuron-specific enolase (NSE) in children with Japanese encephalitis (JE) Methods: Fifty children with JE were selected as observation group. According to Glasgow score, 17 cases were normal group, 20 cases were mild group and 13 cases were severe group. The levels of IL-1β and NSE in cerebrospinal fluid (CSF) and serum during convalescence were detected and compared with those of 20 non-central nervous system diseases. IL-1β was detected by double antibody sandwich enzyme-linked immunosorbent assay, NSE was determined by electrochemiluminescence. Results: The levels of IL-1β and NSE in cerebrospinal fluid (CSF) and serum of children with JE were significantly higher than those of the control group at both the extreme and recovery stages (all P <0.01), and the level of IL-1β and NSE increased in parallel with the severity of illness (P <0.01). There was a positive correlation between cerebrospinal fluid and serum IL-1β and cerebrospinal fluid and serum NSE levels (P <0.01). IL-1β and NSE levels in cerebrospinal fluid and serum IL-1β and NSE levels were also positively correlated (P <0.01). Conclusion: The levels of IL-1β and NSE in cerebrospinal fluid and serum have important reference value for judging the degree of brain injury and clinical condition in children with JE.